10.22
Klik : Genetic transformated Cavendis(NBK)
Genetic transformation of Cavendish banana (Musa spp. AAA group)
cv ‘Grand Nain’ via microprojectile bombardment
Abstract An effective method has been developed for
the stable transformation and regeneration of Caven-
dish banana (Musa spp. AAA group) cv ‘Grand Nain’
by microprojectile bombardment. Embryogenic cell
suspensions were initiated using immature male flowers
as the explant. Cells were co-bombarded with the
neomycin phosphotransferase (nptII) selectable marker
gene under the control of a banana bunchy top virus
(BBTV) promoter or the CaMV 35S promoter, and
either the b-glucuronidase (uidA) reporter gene or
BBTV genes under the control of the maize polyubi-
quitin promoter. Plants were regenerated, under selec-
tion with kanamycin, that were co-transformed with
nptII and either the uidA or BBTV genes. Molecular
characterisation of transformants demonstrated that
the transgenes had been stably integrated into the
banana genome.
Key words Musa 7 Banana 7 Transformation 7 Microprojectile bombardment