Klik : Genetic transformated Cavendis(NBK)

Genetic transformation of Cavendish banana (Musa spp. AAA group)

cv ‘Grand Nain’ via microprojectile bombardment 

Abstract  An effective method has been developed for

the stable transformation and regeneration of Caven-
dish banana (Musa spp. AAA group) cv ‘Grand Nain’
by  microprojectile  bombardment.  Embryogenic  cell
suspensions were initiated using immature male flowers
as  the  explant.  Cells  were  co-bombarded  with  the
neomycin phosphotransferase (nptII) selectable marker
gene under the control of a banana bunchy top virus
(BBTV) promoter or the CaMV  35S promoter, and
either  the  b-glucuronidase (uidA)  reporter  gene  or

BBTV genes under the control of the maize polyubi-
quitin promoter. Plants were regenerated, under selec-
tion  with  kanamycin,  that  were  co-transformed  with
nptII and either the uidA or BBTV genes. Molecular
characterisation  of  transformants  demonstrated  that
the  transgenes  had  been  stably  integrated  into  the
banana genome.


Key words  Musa 7 Banana 7 Transformation 7 Microprojectile bombardment